We found that sciatic-injured rats that were irradiated with LLLT at both 3 and 8 J/cm 2 had significantly improved SFI but that a significant improvement of ROM was only found in rats with LLLT at 8 J/cm 2. Nerve regeneration was investigated by measuring the myelin sheath thickness of the sciatic nerve using transmission electron microscopy (TEM) and by analyzing the expression of growth-associated protein 43 (GAP43) in sciatic nerve using western blot and immunofluorescence staining. Functional assessments of nerve regeneration were analyzed using the sciatic functional index (SFI) and hindlimb range of motion (ROM). Rats were given consecutive transcutaneous LLLT at the crush site and sacrificed 20 days after the crush injury. Thirty-six male Sprague Dawley rats were divided into 6 experimental groups: a normal group with or without 808-nm LLLT at 8 J/cm 2 and a sciatic nerve crush injury group with or without 808-nm LLLT at 3, 8 or 15 J/cm 2.
In this study, we investigated the effective dose of noninvasive 808-nm low-level laser therapy (LLLT) on sciatic nerve crush rat injury model. The development of noninvasive approaches to facilitate the regeneration of post-traumatic nerve injury is important for clinical rehabilitation.
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